Title of R&D Project: Scientifically-Based Concept of Control Measures and Innovative Means for the Prevention of Biological Threats to Ensure National Security (0123U101772)
Project Leader: Tetiana Ivanivna Fotina, Doctor of Veterinary Sciences, Professor
Implementation Period: 01.03.2023 – 31.12.2024
Funding Volume: 1,300,000 UAH
Production trials of the new biocide “Sukhodez” were conducted in floor-raised poultry facilities. A reduction in the number of bacteria, microscopic fungi, and Eimeria oocysts was observed. The hygroscopic properties of the biocide “Sukhodez” were confirmed. The ban on the use of antimicrobial drugs as growth promoters and for the prevention of bacterial infections has led to the search for alternative preventive measures. A production experiment was carried out on the use of probiotic bacterial strains to prevent bacterial infections in broiler chickens, rabbits, and pigs. It was established that probiotics inhibited the growth and reproduction of pathogenic microflora in the intestines, and therefore can be added to animal diets as growth stimulants and biocides. The use of probiotics in dairy cows suffering from ketosis contributed to the restoration of metabolism, productivity, and reduction in ketone levels. Monitoring of the spread and development of preventive measures for the elimination of ectoparasites and bacterial infections in carp before stocking in water bodies was conducted. After treatment with the iodine-containing agent “Kombiyod”, a decrease in contamination by microorganisms and ectoparasites in fish was recorded. As part of the search for a natural and effective component to destroy microorganisms, a study was conducted using high-performance liquid chromatography on eight varieties of Lonicera japonica. The content of phenolic acids in the leaves and branches of the plants was determined. The results were evaluated using multivariate statistical methods such as cluster analysis, comprehensive factor analysis, and partial least squares discriminant analysis (PLS-DA). PLS-DA identified chlorogenic acid, caffeic acid, and ferulic acid as the main components influencing the phenolic acid content in the plants. Antimicrobial peptides can regulate gut microbiota and maintain intestinal homeostasis. It was established that oral administration of the antimicrobial peptide mastoparan in animals had an anti-inflammatory effect and regulated intestinal microbiota.
