Title of R&D Project: Scientifically-Based Concept for the Prevention of Epidemiologically Significant Bacterial Diseases in Animals Using Innovative Technologies (0121U109563)
Project Leader: Tetiana Ivanivna Fotina, Doctor of Veterinary Sciences, Professor
Implementation Period: 01.03.2021 – 31.12.2022
Funding Volume: 1,056.73 thousand UAH
Biocides “Sukhodez” and “Kombiyod” were developed and implemented; these agents destroy microbial biofilms. A feed additive based on chelated compounds and probiotic strains of microorganisms was also created, along with a protocol for its use in animals. An antibacterial peptide MPX was developed, and a bovine mammary epithelial cell model was created to facilitate in vitro experimental studies.
Based on preclinical and clinical studies of environmentally safe veterinary products, informational leaflets were prepared and registration certificates obtained for the developed agents. A veterinary and sanitary strategy for livestock farms was developed using environmentally safe products: “DezSan”, “Sukhodez”, and “Kombiyod”. The use of Bacillus probiotic strains in animals suppresses conditionally pathogenic intestinal microflora and improves metabolism. Monitoring of high-risk diseases in livestock was conducted, and biocides for their prevention were developed. The use of chelated compound complexes in poultry improved the quality of the resulting products. Environmentally safe antiprotozoal agents for livestock were developed.
Research demonstrated the therapeutic effect of Bacillus megaterium NCH 55 in subclinical mastitis in cows. The issue of mycotoxicosis in cows was studied, and highly sensitive and specific monoclonal antibodies against ZEN (mAb) were developed for detecting ZEN residues in food and feed. The quality of goat milk was studied under organic livestock farming conditions. The current environmental situation in Ukraine is a crisis that has evolved over a long period due to disregard for laws related to the development and reproduction of the state’s natural resource system, particularly the unjustified and uncontrolled use of antimicrobial agents.
A multiplex PCR assay was developed for the identification of Salmonella Pullorum among 33 different Salmonella enterica subsp. enterica and 13 non-target species. Oral administration of MPX reduced the mRNA expression of inflammation-related factors, thereby alleviating intestinal inflammation caused by E. coli infection in mice. Studies also confirmed the activity of MPX against S. aureus.
